Preparation of heparin-functionalized microspheres and study on their adsorption characteristic for basic protein lysozyme

Jiying Men; Ruixin Wang; Xiaoyu Hu; Hongyu Zhao; Hongwei Wei; Cong Hu; Baojiao Gao

Macromolecular Research, Vol.24, No.2, 114-122, February, 2016

DOI10.1007/s13233-016-2016-6 Export Citation

Preparation of heparin-functionalized microspheres and study on their adsorption characteristic for basic protein lysozyme

Jiying Men^†, Ruixin Wang, Xiaoyu Hu, Hongyu Zhao, Hongwei Wei, Cong Hu, and Baojiao Gao

Department of Chemical engineering, North University of China, Taiyuan, 030051, P. R. China

E-mail:

In suspension polymerization system, poly(glycidyl methacrylate) (PGMA) microspheres are firstly prepared. Then, ring opening reaction takes place when heparin is bonded on PGMA microspheres to obtain functional PGMA-heparin microspheres. The chemical structure and physicochemical characters of PGMA-heparin microspheres are fully characterized with infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and zeta potential determination. The adsorption properties of PGMA-heparin for lysozyme are mainly investigated. The effects of the main factors on the adsorption properties are explored, furthermore the adsorption mechanism is analyzed in depth, and the adsorption thermodynamics is also researched. The experimental results show that the strong electrostatic interaction is formed between high density of negative charge coming from the carboxyl groups or sulfonic acid groups of heparin and the positive charge of basic protein lysozyme. The adsorption of PGMA-heparin for lysozyme is dependent on the pH value of the medium, and the saturated adsorption amount of PGMA-heparin for lysozyme possesses a maximum at pH 8, which reaches up to 654 mg/g. The adsorption of PGMA-heparin for lysozyme is an exothermic process which is driven by entropy, and the adsorption amount decreases with increasing the temperature.

Keywords:heparin;lysozyme;adsorption;low density lipoprotein (LDL)

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[Referenced By]

[1] Couzin J, Science, 322, 220 (2008)

[2] Fox KM, Gandhi SK, Bullano MF, Ohsfeldt RL, Davidson MH, Circulation, 117, E425 (2008)

[3] Chamberlain AM, Folsom AR, Schreiner PJ, Boerwinkle E, Ballantyne CM, Atherosclerosis, 200, 322 (2008)

[4] Shoji T, Hatsuda S, Tsuchikura S, Shinohara K, Kimoto E, Koyama H, Emoto M, Nishizawa Y, Atherosclerosis, 202, 582 (2009)

[5] Bambauer R, Schiel R, Latza R, Ther. Apher. Dial., 7, 382 (2003)

[6] Bosch T, Ther. Apher. Dial., 9, 459 (2005)

[7] Liu DB, He BJ, Han SY, Wang SQ, Liu QP, ichi AJ, Osa T, Chen Q, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 27, 665 (2007)

[8] Stegmayr BG, Transfus. Apher. Sci., 32, 209 (2005)

[9] Soltys PJ, Etzel MR, Biomaterials, 21, 37 (2000)

[10] Li HT, Zhang YM, Chen XF, Shi KY, Yuan Z, Liu B, Shen B, He BL, React. Funct. Polym., 58(1), 53 (2004)

[11] Wang SQ, Yu YT, Cui T, Cheng Y, Biomaterials, 24, 2799 (2003)

[12] Bosch T, Schmidt B, Kleophas W, Gillen C, Otto V, Passlick-Deetjen J, Gurland HJ, Artif. Organs, 21, 977 (1997)

[13] Borberg H, Transfus. Apher. Sci., 41, 49 (2009)

[14] Wang W, Huang XJ, Cao JD, Lan P, Wu W, Acta BiolnKmater., 10, 234 (2014)

[15] Zhao LR, Sun DX, Liu MY, Carbohydr. Polym., 78, 828 (2009)

[16] Huang XJ, Guduru D, Xu ZK, Vienken J, Groth T, Acta Biomater., 6, 1099 (2010)

[17] Ma KW, Ma L, Cai SX, Wang X, Liu B, Xu ZL, J. Mater. Sci. -Mater. Med., 19, 3255 (2008)

[18] Zheng XM, Huang XJ, Xu ZK, Acta Polym. Sin., 7, 791 (2011)

[19] Kim SE, Kim CS, Yun YP, Yang DH, Park K, Kim SE, Jeong CM, Huh JB, Carbohydr. Polym., 114, 123 (2014)

[20] Yu S, Yu ZT, Wang G, Dargusch MS, Zhang MH, Rare Metal Mater. Eng., 38, 0384 (2009)

[21] Deng Y, Sun YH, Chen XF, Zhu PZ, Wei SC, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 3, 2905 (2013)

[22] Buzoglu L, Maltas E, Ersoz M, Yildiz S, React. Funct. Polym., 82, 25 (2014)

[23] Sun YL, Yan YW, J. Control. Release, 172, E14 (2013)

[24] Abd El-Ghaffar MA, Hashem MS, El-Awady MK, Rabie AM, Carbohydr. Polym., 89, 667 (2012)

[25] Li F, Ma M, Yuan ZF, Huang JY, Zhuo RX, J. Bioact. Compat. Polym., 26, 388 (2011)

[26] Christine TS, Ipsita AB, Materials, 2, 577 (2009)

[27] Li PF, Yang LR, He XQ, Wang J, Kong P, Xing HF, Liu HZ, Chin. J. Chem. Eng., 20(1), 95 (2012)

[28] Yu YL, Gao BJ, Li YF, Chin. J. Catal., 34, 1776 (2013)

[29] Tong Z, Silicone Mater., 26, 258 (2012)

[30] Huang XJ, Guduru D, Xu ZK, Vienken J, Groth T, Acta Biomater., 6, 1099 (2010)

[31] Gao BJ, Fu HY, Li YB, Du RK, J. Chromatogr. B, 878, 1731 (2010)

[32] Guo HY, Wu YM, Yuan J, Yang FF, Sep. Sci. Technol., 49(16), 2548 (2014)

[33] Cestari AR, Vieira EFS, Mattos CRS, J. Chem. Thermodyn., 38(9), 1092 (2006)

[34] dos Anjos FSC, Vieira EFS, Cestari AR, J. Colloid Interface Sci., 253(2), 243 (2002)

[35] Cestari AR, Vieira EFS, Mattos CRS, J. Chem. Thermodyn., 38(9), 1092 (2006)

[36] Zhang YY, Gao BJ, Xu ZQ, J. Phys. Chem. B, 117(18), 5730 (2013)