화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.33, No.2, 610-615, February, 2016
DOI10.1007/s11814-015-0190-z  Export Citation
A facile technique to prepare cross-linked enzyme aggregates of bovine pancreatic lipase using bovine serum albumin as an additive
Jian Dong Cui1, 2, †, Rong Lin Liu1, 3, and Lin Bo Li1, 3
  • 1Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 70 Yuhua East Road, Shijiazhang 050018, P. R. China
  • 2Tianjin Key Laboratory of Food-Biotechnology, Tianjin University of Commerce, Beichen District, Tianjin 300134, P. R. China
  • 3, China
E-mail:
Cross-linked enzyme aggregates from bovine pancreatic lipase were prepared by co-aggregation of lipase and BSA (Lipase-BSA-CLEAs). The main factors in the preparation of lipase-BSA-CLEAs were optimized. The highest activity recovery was around 75% under the condition of using 1% (v/v) glutaraldehyde as cross-linker and 0.05 g/L bovine serum albumin as feeder for 2 h cross linking. The optimum temperature for both lipase-CLEAs and lipase-BSA-CLEAs was measured as 60 oC, which is 10 oC higher than that of free lipase. Moreover, the lipase-BSA-CLEAs evidenced higher thermal stability and excellent reusability in comparison with the lipase-CLEAs. Lipase-BSA-CLEAs retained more than 75% of the initial activity after eight cycles of reuse, while lipase-CLEAs only retained 20% of its initial activity. Additionally, lipase-BSA-CLEAs showed more storage stability than free lipase and lipase-CLEAs. The high stability and recyclability of lipase-BSA-CLEAs make it efficient for different industrial applications.
Keywords:Bovine Pancreatic Lipase;Cross-linked Enzyme Aggregates;Immobilization Enzyme;BSA
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