화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.40, No.1, 85-91, January, 2016
DOI10.7317/pk.2016.40.1.85  Export Citation
비타민 C를 함유한 락타이드 글리콜라이드 공중합체 지지체에서의 항염증, 항산화 효과
Inflammatory Response and Antioxidation on Vitamin C Impregnated Poly(lactide-co-glycolide) Scaffold
장나금, 이선의, 차세롬, 김창현, 송정은, 권순용1, 강길선†
  • 전북대학교 BIN융합공학과, 고분자나노공학과, 고분자융합소재연구소
  • 1가톨릭대학교 의과대학 여의도성모병원 정형외과
Na Keum Jang, Seon Eui Lee, Se Rom Cha, Chang Hyun Kim, Jeong Eun Song, Soon Yong Kwon1, and Gilson Khang†
  • Dept. of BIN Fusion Tech & Dept. of Polymer Nano Sci. Tech., Chonbuk National University, 567 Baekje-daero, Jeonju 54898, Korea
  • 1Dept. of Orthepedic Surgery, Yeouido St. Mary’s Hospital, Catholic University of Korea, 62 Yeouido-dong, Yeongdeungpo-gu, Seoul 07345, Korea
E-mail:
초록
Poly(lactide-co-glycolide) (PLGA)는 대표적인 생분해성, 생체적합성을 가진 고분자로 조직공학 및 약물전달체로 널리 적용되고 있다. 그러나 이식 후에 PLGA는 에스테르결합으로 인해 가수분해가 일어나 분해산물의 영향으로 pH가 낮아져 조직에 염증반응을 유발한다. 비타민 C는 보통 식품, 약품에서 사용되고 있고, 강력한 항산화와 항염증 효과를 나타내는 것으로 알려져 있다. 그래서 조직공학 전달체로서의 PLGA와 비타민 C를 연구하였다. 본 연구에서는, 비타민 C가 포함된 PLGA 지지체를 제작하여 비타민 C의 생체 외 염증반응을 평가하였다. 지지체는 PLGA 무게당 3, 5, 10 wt%의 비타민 C를 첨가하여 캐스팅/염 추출법으로 3차원 비타민 C/PLGA 지지체로 제작하였다. 지지체의 물리적 특성은 압축강도와 SEM을 통해 조사하였다. 세포의 부착과 증식률은 MTT와 SEM 분석을 통해 실험하였다. 또한 RT-PCR과 FACS를 통해 항염증, 항산화 효과를 확인하였다. 결과는 공통적으로 5 wt% 비타민 C/PLGA 지지체가 항산화, 항염증 효과, 세포의 증식률의 면에서 다른 지지체보다 우수한 것으로 나타났다. 따라서 항염증 효과를 기초로하여, 5 wt% 비타민 C/PLGA 지지체는 미래의 조직공학에 쓰일 생체재료로 적용될 수 있을 것으로 사료된다.
Typical biodegradable polymer poly(lactide-co-glycolide) (PLGA) has been widely applied to tissue engineering scaffolds and drug delivery systems. However, after the implantation, the ester bond of PLGA gets hydrolyzed and the resulting degradation products decrease the pH in the surrounding tissue causing local inflammatory reaction. Vitamin C is commonly used in food, pharmaceutical products and is known to exert potent antioxidant and anti-inflammatory activities. Thus, vitamin C is used with PLGA as carrier in tissue engineering. In this study, vitamin C incorporated PLGA scaffolds were fabricated and further the effects of vitamin C were evaluated on the inflammatory responses in vitro. The three-dimensional PLGA scaffolds were fabricated by adding of 3, 5, and 10 wt% vitamin C via a casting extraction method. Then the samples were examined; the mechanical properties of compressive strength and SEM. The cell attachment and proliferation were also studied the MTT, SEM, anti-inflammatory and antioxidant effects by RT-PCR and FACS. Results showed that 5 wt% vitamin C/PLGA scaffold was better than other scaffolds in terms of anti-inflammatory, antioxidant, cell attachment and proliferation. Based on the low level of inflammatory responses, the 5 wt% vitamin C/PLGA scaffold can be envisioned as a promising candidate for future biomedical applications.
Keywords:PLGA;vitamin C;scaffold;inflammation;ROS
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