다양한 용매에서 Soxhlet 추출기를 이용한 감귤 껍질에서 d-limonene 추출의 최적조건 연구

박상민; 고관영; 김인호; Sang Min Park; Kwan Young Ko; In Ho Kim

Korean Chemical Engineering Research, Vol.53, No.6, 717-722, December, 2015

DOI10.9713/kcer.2015.53.6.717 Export Citation

다양한 용매에서 Soxhlet 추출기를 이용한 감귤 껍질에서 d-limonene 추출의 최적조건 연구

Optimization of d-limonene Extraction from Tangerine Peel in Various Solvents by Using Soxhlet Extractor

박상민, 고관영, 김인호^†

충남대학교 화학공학과, 34134 대전광역시 유성구 대학로 99

Sang Min Park, Kwan Young Ko, and In Ho Kim^†

Department of Chemical Engineering, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea

E-mail:

초록

감귤류의 과일 내에 포함된 것으로 알려진 d-limonene 성분은 정유 성분을 추출하여 분리하는 방법으로 얻을 수 있다. 연속 증류 추출법 중 하나인 Soxhlet 추출법을 사용하여 감귤 껍질의 정유 성분을 추출한 후, 역상 HPLC를 통해 정량분석을 하였다. 추출용매의 끓는점과 극성도가 수율에 주는 영향을 알아보기 위해 에탄올, n-헥산, 석유에테르 3가지 용매를 추출 용매로 사용하였다. 120 oC에서 각각의 용매를 1, 2, 4시간 동안 추출했을 때의 시료를 얻어 총 9가지 시료를 분석하였다. 역상 HPLC에서 정량분석을 한 결과, 세 가지 용매 모두 2시간 동안 추출했을 때 d-limonene의 함유량이 가장 많았으며, 이 때 감귤 껍질 1 g당 함유량은 7.77 mg(에탄올), 0.49 mg(n-헥산), 0.28 mg(석유에테르)였다. 에탄올을 사용했을 때 수율이 가장 높은 것으로 보아 용매의 극성도가 끓는점보다 추출 효율에 더 큰 영향을 미치는 것을 알 수 있었다.

D-limonene included in citrus fruits is obtainable to extract essential oil as well as separate the oil ingredient. Soxhlet extraction, a type of SDE (Simultaneous steam Distillation and solvent Extraction), was used to extract limonene from tangerine peel. HPLC analysis was performed to quantify extracted d-limonene by using reversed-phase HPLC column. Results of HPLC analysis showed that the optimal extraction time was 2 hours in any solvent, and the extracted amounts of d-limonene in tangerine peel (per g tangerine peel) were 7.77 mg, 0.49 mg, and 0.28 mg in ethyl alcohol, nhexane, and ether. Because yield was the highest in using ethyl alcohol as a solvent, polarity is stronger factor to effect on yield of extraction than boiling point.

Keywords:Tangerine Peel;d-limonene;Soxhlet Extraction;HPLC

[References]

Morse MA, Toburen AL, Cancer Lett., 104(2), 211 (1996)
Budavari S, The Merck Index, 7th ed., Merck, New Jersey(1989).
Langer RS, Wise DL, Medical Applications of Controlled Release, 2nd ed., CRC Press, Florida(2004).
Rosen HB, Chang J, Wnek GE, Linhardt RJ, Langer R, Biomaterials, 4(2), 131 (1983)
Oh EH, Im HS, Yoon CH, J. Korean Oil Chemists' Society, 26(3), 350 (2009)
Im HH, “Studies on Extraction of Limonene from Citron and Immunomodulation Activity of Limonene,” M.S. Thesis, Hankyong National University, Ansung, Korea(2005).
Beak JI, Jin SY, Kim IH, “Separation of α-lactalbumin and β-lactoglobulin by Using HPLC,” B. S. Thesis, Chungnam National University, Daejeon, Korea(2009).
Wixom RL, Chromatography, Wiley Press, New York(2011).
Kim JI, Choi DY, Row KH, Korean J. Chem. Eng., 20(3), 538 (2003)
Nilsson U, Bergh M, Shao LP, Karlberg AT, Chromatographia, 42(3-4), 199 (1996)
Zhang RL, Zhang Q, Xu SA, J. Anhui Agri. Sci., 13(2), 743 (2012)
http://www.sanderkok.com/techniques/hplc/eluotropic_series_extended.html.
Yoon CH, Bok HS, Choi DK, Row KH, Korean Chem. Eng. Res., 50(3), 545 (2012)
Lee KJ, Choi DY, Row KH, Korean Chem. Eng. Res., 41(5), 612 (2003)

[1] Morse MA, Toburen AL, Cancer Lett., 104(2), 211 (1996)

[2] Budavari S, The Merck Index, 7th ed., Merck, New Jersey(1989).

[3] Langer RS, Wise DL, Medical Applications of Controlled Release, 2nd ed., CRC Press, Florida(2004).

[4] Rosen HB, Chang J, Wnek GE, Linhardt RJ, Langer R, Biomaterials, 4(2), 131 (1983)

[5] Oh EH, Im HS, Yoon CH, J. Korean Oil Chemists' Society, 26(3), 350 (2009)

[6] Im HH, “Studies on Extraction of Limonene from Citron and Immunomodulation Activity of Limonene,” M.S. Thesis, Hankyong National University, Ansung, Korea(2005).

[7] Beak JI, Jin SY, Kim IH, “Separation of α-lactalbumin and β-lactoglobulin by Using HPLC,” B. S. Thesis, Chungnam National University, Daejeon, Korea(2009).

[8] Wixom RL, Chromatography, Wiley Press, New York(2011).

[9] Kim JI, Choi DY, Row KH, Korean J. Chem. Eng., 20(3), 538 (2003)

[10] Nilsson U, Bergh M, Shao LP, Karlberg AT, Chromatographia, 42(3-4), 199 (1996)

[11] Zhang RL, Zhang Q, Xu SA, J. Anhui Agri. Sci., 13(2), 743 (2012)

[12] http://www.sanderkok.com/techniques/hplc/eluotropic_series_extended.html.

[13] Yoon CH, Bok HS, Choi DK, Row KH, Korean Chem. Eng. Res., 50(3), 545 (2012)

[14] Lee KJ, Choi DY, Row KH, Korean Chem. Eng. Res., 41(5), 612 (2003)