화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.39, No.6, 940-946, November, 2015
DOI10.7317/pk.2015.39.6.940  Export Citation
Poly(p-phenyleneethynylene)계 수용성 공액화 고분자: 합성과 세포 이미징
Water-soluble Conjugated Poly(p-phenyleneethynylene)s: Synthesis and Cell Imaging
김종호1, 최문수1, 2, Jinsang Kim3, 이택승1, †
  • 1충남대학교 유기소재 · 섬유시스템공학과
  • 2전자재료사업부 삼성 SDI
  • 3University of Michigan Department of Materials Science and Engineering
Jongho Kim1, Moon Soo Choi1, 2, Jinsang Kim3, and Taek Seung Lee1, †
  • 1Organic and Optoelectronic Materials Laboratory, Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon 305-764, Korea
  • 2Marketing Team, Electronic Materials Division, Samsung SDI, Metan-dong 560, Youngtong-gu, Suwon, Gyeonggi-do 443-803, Korea
  • 3Department of Materials Science and Engineering, University of Michigan, Ann Arbor, Michigan 48109, U. S. A., USA
E-mail:
초록
최근 수용성 형광 공액화 고분자를 이용한 세포 형광 이미징을 활용하여 생체물질의 정량 정성 분석에 대한 연구분야는 많은 관심을 끌고 있다. 이를 위하여 주사슬에 벤조싸이아다이아졸을 포함하는 poly(p-phenyleneethynylene)(PPE)형 수용성 형광 공액화 고분자를 합성하여, 주사슬 내 벤조싸이아다이아졸의 함량에 따라 수용액에서 청색부터 적색 형광까지 나타나는 형광 고분자를 얻었다. 이러한 PPE형 형광 공액화 고분자는 수용성을 갖기 위하여 고분자 곁사슬에 비이온성기인 올리고(에틸렌 글리콜)이 도입되었으며, 또한 올리고(에틸렌 글리콜)기 말단에 카르복실 산기를 도입하였다. 이 카르복실 산기는 세포와 상호작용할 수 있는 단백질 마커와 공유결합을 형성하였으며, 그 결과 형광 공액화 고분자를 사용한 선택적 세포 이미징이 성공적으로 수행되었다.
We report a synthesis of water-soluble poly(p-phenyleneethynylene)(PPE) copolymers containing 2,1,3-benzothiadiazole for cell imaging. The polymers showed emission ranges from blue to red fluorescence according to their benzothiadiazole contents in the backbones. One of the nonionic groups, oligo(ethylene glycol) unit was incorporated in the side chain of the polymers to enhance water-solubility of the polymers. Carboxylic acid groups were introduced at the end of the oligo(ethylene glycol) unit for the specific interaction with cells. As a result, specific cell imaging was successfully accomplished using the red-emitting, water-soluble conjugated polymers containing benzothiadiazole.
Keywords:poly(p-phenyleneethynylene);water-soluble conjugated polymers;cell imaging;fluorescence
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