오리발 유래 콜라겐/락타이드 글리콜라이드 하이브리드 지지체의 특성 및 염증 완화 효과

김수민; 김혜민; 국현; 김은영; 송정은; 서동삼; 박찬흠; 강길선; Soo Min Kim; Hye Min Kim; Hyeon Kuk; Eun Young Kim; Jeong Eun Song; Dong Sam Suh; Chan Hum Park; Gilson Khang

Polymer(Korea), Vol.39, No.6, 837-845, November, 2015

DOI10.7317/pk.2015.39.6.837 Export Citation

오리발 유래 콜라겐/락타이드 글리콜라이드 하이브리드 지지체의 특성 및 염증 완화 효과

Characterization and Effect of Inflammatory Reaction of Duck-Feet Derived Collagen/PLGA(Poly lactic-co-glycolide) Hybrid Scaffold

김수민, 김혜민, 국현, 김은영, 송정은 , 서동삼¹, 박찬흠 ², 강길선 ^†

BIN 융합공학과, 고분자나노공학과, 고분자 BIN융합 연구소 전북대학교
¹세원셀론텍
²한림대학교 의과대학 춘천성심병원 이비인후과

Soo Min Kim, Hye Min Kim, Hyeon Kuk, Eun Young Kim, Jeong Eun Song , Dong Sam Suh¹, Chan Hum Park², and Gilson Khang^†

Dept. of BIN Fusion Tech, Polymer BIN Fusion Res Center & Dept. of PolymerNano Sci Tech, Chonbuk National Univ., 567 Deokjin, Jeonju, Chonbuk 561-756, Korea
¹Sewon Cellontech, 801, Wooyoung techno center, 273-15 Seongsu 2ga 3-dong, Seongdong gu, Seoul 133-831, Korea
²Dept. of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, 1-1 Okcheon, Chuncheon, Gangwon 200-702, Korea

E-mail:

초록

본 연구에서는 오리발에서 추출한 콜라겐(extracted from feet of ducks)을 함유한 poly(lactic-co-glycolide) (PLGA) 복합 지지체를 제조하여 조직 공학적으로 적용될 수 있도록 지지체의 기본 특성과 염증 반응을 알아보고자 하였다. 그 특성을 알아보기 위하여 압축강도, SEM, MTT를 측정하였으며, RT-PCR, ROS 그리고 조직화학적 염색을 통해 염증반응을 확인하였다. 그 결과 DC/PLGA 지지체는 기계적 성질 및 증식이 우수하고 염증반응 또한 완화시켜 줄 수 있음을 알 수 있었다. 이로써, DC가 첨가된 PLGA 지지체가 조직공학적인 지지체로 적용될 가능성을 확인하였다.

In this study, hybrid scaffold composed of duck’s feet extract (DC)/poly(lactic-co-glycolide) (PLGA) were fabricated to apply in tissue engineering. Characterizations such as compressive strength, SEM analysis and MTT were performed for the analysis of basic properties. RT-PCR, ROS and H&E staining were carried out for anti-inflammatory effect. DC/PLGA scaffolds have shown excellent mechanical properties and proliferation and can reduce inflammatory response. This study suggested that DC/PLGA scaffolds may serve as a potential cell delivery vehicle and a structural basis for tissue engineering.

Keywords:collagen;feet of duck;inflammatory reaction;scaffold;tissue engineering

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[1] Deyl Z, Adam M, J. Chromatography, 488, 161 (1989)

[2] Ko HA, Kim HM, Ha HJ, Kim AR, Jang JE, Kim KH, Lee JH, Song JE, Yoon H, Lee OJ, Lee D, Park CH, Khang G, Int. J. Tissue Regen., 3, 102 (2012)

[3] Deyl Z, Miksik, Eckhardt A, J. Chromatogr. B, 790, 245 (2003)

[4] Liu C, Xia Z, Czernuszka JT, Chem. Eng. Res. Des., 85(A7), 1051 (2007)

[5] Pachence JM, J. Biomed. Mater. Res., Part B: Appl. Biomater., 33, 35 (1996)

[6] Santos MH, Silva RM, Dumont VC, Neves JS, Mansur HS, Heneine LGD, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 33, 790 (2013)

[7] Caputo I, Lepretti M, Scarabino C, Esposito C, Proto A, Anal. Biochem., 421, 92 (2012)

[8] Zeugolis DI, Paul RG, Attenburrow G, J. Biomed. Mater. Res. A, 86, 892 (2008)

[9] Deo V, Gupta S, Ansari S, Kumar P, Yadav R, Quintessence Int., 45, 15 (2014)

[10] Maspero F, Sala S, Fedi ME, Martini M, Papagni A, Anal. Bioanal. Chem., 401, 2019 (2011)

[11] Feng W, Zhao T, Zhou Y, Li F, Zou Y, Bai S, Wang W, Yang L, Wu X, Pharmacogn. Mag., 9, 32 (2013)

[12] Fagerholm P, Lagali NS, Ong JA, Merrett K, Jackson WB, Polarek JW, Suuronen EJ, Liu Y, Brunette I, Griffith M, Biomaterials, 35, 2420 (2014)

[13] Shen ZL, Kahn H, Ballarini R, Eppell SJ, Biophys. J., 100, 3008 (2011)

[14] Ghezzi CE, Marelli B, Muja N, Nazhat SN, Acta Biomater., 8, 1813 (2012)

[15] El-Fiqi A, Lee JH, Lee EJ, Kim HW, Acta Biomater., 9, 9508 (2013)

[16] Sopakayang R, De Vita R, Kwansa A, Freeman JW, J. Theor. Biol., 293, 197 (2012)

[17] Khang G, Kim MS, Cho SH, Lee I, Rhee JM, Lee HB, Tissue Eng. Regen. Med., 1, 34 (2004)

[18] Yoshimoto H, Shin YM, Terai H, Biomaterials, 24, 2077 (2003)

[19] Fan H, Hu Y, Zhang C, Li X, Lv R, Qin L, Zhua R, Biomaterials, 27, 4573 (2006)

[20] Wu YC, Shaw SY, Lin HR, Lee TM, Yang CY, Biomaterials, 27, 896 (2006)

[21] Goh SK, Bertera S, Olsen P, Candiello JE, Halfter W, Uechi G, Balasubramani M, Johnson SA, Sicari BM, Kollar E, Badylak SF, Banerjee I, Biomaterials, 34, 6760 (2013)

[22] Lu H, Hoshiba T, Kawazoe N, Koda I, Song M, Chen G, Biomaterials, 32, 9658 (2011)

[23] Eom S, Park JH, Yoo SC, Kim SJ, Khang G, Int. J. Tissue. Regen., 1, 28 (2010)

[24] Curran JM, Fawcett S, Hamilton L, Rhodes NP, Rahman CV, Alexander M, Shakesheff K, Hunt JA, Biomaterials, 34, 9352 (2013)

[25] Lee SJ, Int. J. Tissue Regen., 4, 89 (2013)

[26] Chung TJ, Lee BH, Lee HJ, Kwon HJ, Jang WB, Kim HJ, Eom YG, Elastomer, 46, 195 (2011)

[27] Kim HE, Kim HN, Yu H, Song JE, Jeoung SY, Kim Y, Lee D, Khang G, Macromol. Res., 20(10), 1044 (2012)

[28] Ngiam M, Liao S, Patil AJ, Cheng Z, Chan CK, Ramakrishna S, Bone, 45, 4 (2009)

[29] Farrugia BL, Whitelock JM, Jung M, McGrath B, O'Grady RL, McCarthy SJ, Lord MS, Biomaterials, 35, 1462 (2014)

[30] Ashley RA, Palmer BW, Schultz AD, Woodson BW, Roth CC, Routh JC, Fung KM, Frimberger D, Lin HK, Kropp BP, Tissue Eng. Part A, 15, 3241 (2009)

[31] Bucktrout SLB, Llordella MM, Zhou X, Anthony B, Rosenthal W, Luche H, Fehling HJ, Bluestone JA, Immunity, 39, 949 (2013)

[32] Abouzid K, Bekhit SA, Bioorg. Med. Chem., 16, 5547 (2008)