화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.25, 145-150, May, 2015
DOI10.1016/j.jiec.2014.10.026  Export Citation
Optimization of medium composition for enhanced cellulase production by mutant Penicillium brasilianum KUEB15 using statistical method
Da Un Jung, Hah Young Yoo, Sung Bong Kim, Ja Hyun Lee, Chulhwan Park1, and Seung Wook Kim†
  • Department of Chemical and Biological Engineering, Korea University, Seoul, 136-701, Republic of Korea
  • 1Department of Chemical Engineering, Kwangwoon University, Wolgye-Dong, Nowon-Gu, Seoul, Republic of Korea
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Penicillium brasilianum KUEB15 was utilized for cellulase production and medium optimization was investigated. The optimal compositions of the variables for maximum CMCase production by central composite rotatable design were 12.39 g/L of corn stover, 0.48 g/L of urea, and 6.12 g/L of ammonium sulfate. The predicted CMCase activity by the model was 1.207 Unit/ml, and the observed CMCase activity which was produced in optimal media was 1.180 Unit/ml. The activity of CMCase from P. brasilianum KUEB15, which was fermented in optimal media by RSM, was found to be 1.61-fold increased than that in the Mandel's medium (0.731 Unit/ml).
Keywords:Penicillium brasilianum;Cellulase;Optimization;Plackett-Burman design;Central composite rotatable design
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