Nalidixic acid imprinted polymer has high application potential in clinical purposes. Molecularly imprinted polymers were prepared using nalidixic acid as the template molecule. The polymer was synthesized in a non-covalent approach with N,N-dimethylacrilamid and allyl glycidyl ether/iminodiacetic acid as the functional monomer. The effects of various parameters such as pH and adsorption time on adsorption capacity were considered. It was confirmed that the binding ability of the prepared molecularly imprinted polymer for nalidixic acid was essentially sufficient in the presence of the other compounds coexisting in human urine. Color versions of one or more of the figures in the article can be found online at [GRAPHICS] .