Separation of trivalent actinides and lanthanides is a challenging task and has a great relevance in the nuclear fuel cycle. Bis(2,4,4-trimethylpentyl)dithiophosphinic acid (Cyanex-301) show high selectivity for the trivalent actinides over the lanthanides at pH 3 or higher and N-donor ligands were reported to enhance the selectivity. 2,2MODIFIER LETTER PRIME:6MODIFIER LETTER PRIME,6"-Terpyridine (terpy), on the other hand, has shown to be quite effective at lower pH values and the combination of Cyanex 301 and terpy was evaluated in the present study, for the first time, for the separation of Am(III) from Eu(III), representative actinide and lanthanide elements, respectively at pH 2.0. Thermodynamic parameters (enthalpy, entropy, and free energy) for the two phase extraction were also determined from the distribution studies at variable temperatures. Extraction of both Am3+ and Eu3+ was favored by negative enthalpy of extraction. More negative Delta G value indicated that Am3+ extraction was more favoured as compared to Eu3+ extraction using this solvent system. Effect of diluent composition on the extraction of Am3+ and Eu3+ was also studied in the present work.