We constructed expression vectors for Thermus thermophilus. The transformation efficiency of pTEV131 per viable cells was 0.15% at 60 degrees C and 0.11% at 68 degrees C, and with marker rescue transformation, it was 1.9% at 60 degrees C and 0.5% at 68 degrees C. When the crtB gene was inserted into the cloning sites of this vector, the transformant expressed 3-fold more crtB gene than the wild type.