A series of dimethylaminopropyl benzyl chloride grafted polysiloxanes (PDMS-g-BCs) with tunable molecular weights and cationic content were synthesized, and the effect of polymer structure on the antimicrobial activities against bacteria (Escherichia coli (E. coli) and Staphylococcus albus (S. albus)) and phytopathogenic fungi (Rhizoctonia solani (R. solani) and Fusarium oxysporum f. sp. cubense race 4 (Foc4)) were systematically assessed. The antibacterial activity was evaluated by determining the minimum inhibitory concentration (MIC) against E. coli and S. albus by the broth dilution method, and the antifungal activity was measured by mycelia growth inhibition as well as by the MIC and minimum fungicidal concentration (MFC) values. The molecular weight and cationic content were major determinants of the activities of PDMS-g-BCs; when the molecular weight was approximately 2500 g/mol and the cationic content was approximately 20 mol% of the total siloxane units, PDMS-g-BCs gained strong antimicrobial activities toward both E. coli and Foc4, comparable to those of a commonly used broad-spectrum microbicide benzalkonium chloride, making PDMS-g-BCs promising fungicidal agents for plant disease control. (C) 2014 Elsevier B.V. All rights reserved.