Production of a Recombinant Human Monoclonal-Antibody Using a Novel Hollow-Fiber Bioreactor System

Inoue Y; Kawamoto S; Seki K; Teruya K; Mochizuki K; Kato M; Hashizume S; Yasumoto K; Nagashima A; Nakahashi H; Suzuki T; Imai T; Nomoto K; Shirahata S

Journal of Fermentation and Bioengineering, Vol.81, No.5, 466-469, 1996

DOI10.1016/0922-338X(96)85150-6 Export Citation

Production of a Recombinant Human Monoclonal-Antibody Using a Novel Hollow-Fiber Bioreactor System

Inoue Y, Kawamoto S, Seki K, Teruya K, Mochizuki K, Kato M, Hashizume S, Yasumoto K, Nagashima A, Nakahashi H, Suzuki T, Imai T, Nomoto K, Shirahata S

The amplified ras oncogene was used to enhance the productivity of BHK-21 cells producing a lung cancer-reactive human recombinant AE6F4 monoclonal antibody. The recombinant antibody (18 mg) was produced by the ras-amplified BHK-21 cells in a one-month high-density culture using the novel hollow fiber bioreactor system Tecnomouse.

Keywords:LUNG-CANCER;FUTURE