Single crystals of Di-Leucine hydrochloride (DLHC) were grown by slow evaporation at constant temperature (40 degrees C). The compound crystallizes in the noncentrosymmetric space group P2(1). The physicochemical properties of the grown crystal were characterized by infrared spectroscopy, thermal and optical analysis, measurement of hardness. The second harmonic generation efficiency of the sample was investigated and it was found to be 1.3 times of KDP. (C) 2014 Elsevier B.V. All rights reserved.