Enhancement of Mutation Frequency with Nucleotide Triphosphate Analogs in PCR Random Mutagenesis

Ueda H; Nakanishi M; Suzuki E; Nagamune T

Journal of Fermentation and Bioengineering, Vol.79, No.3, 303-305, 1995

DOI10.1016/0922-338X(95)90624-9 Export Citation

Enhancement of Mutation Frequency with Nucleotide Triphosphate Analogs in PCR Random Mutagenesis

Ueda H, Nakanishi M, Suzuki E, Nagamune T

The effects of the deoxynucleotide triphosphate analogs 2’-deoxyinosine 5’-triphosphate and 5-bromo-2’-deoxyuridine 5’-triphosphate on the enhancement of random mutation in PCR were investigated. Addition of these analogs was found to be an effective and easy method for making various mutant DNA sequences of lacZ’.

Keywords:DNA-POLYMERASE;FIDELITY