We report novel vesicles composed of the zwitterionic surfactant lauryl sulfobetaine (LSB), which is a simple single-tailed surfactant (STS). The novel vesicles spontaneously formed from LSB micellar solutions with the mediation of a rough glass surface (RGS) in the absence of any cosurfactants or additives. Importantly, the obtained STS vesicles displayed good stability upon long-term storage, exposure to high temperature, and freeze-thawing after the RGS was removed. The pH of the LSB solution (4.0-9.0) and the presence of NaCl (1.0 X 10(-5)) and 1.0 X 10(-4) mol/L) in the LSB solution had no obvious influence on the formation and stability of the vesicles. The adsorption configuration of LSB on the RGS was investigated via water contact angle measurements and atomic force microscope observations. The results showed that LSB adsorption bilayers could form on the RGS, and the bilayer adsorption of LSB on the RGS and the roughness of the solid surface played a key role in the vesicle formation. A possible mechanism for the RGS-mediated formation of LSB vesicles is proposed: LSB micelles and molecules adsorb on the RGS to form curved bilayers, and the curved bilayers are then detached from the RGS and close to form vesicles. To the best of our knowledge, this is the first report of LSB alone forming vesicles. This finding extends our understanding of the nature of vesicle systems.