Significant specific pseudosolubilizing activity of the n-hexadecane pseudosolubilizing factor (C16PSF), n-octadecane pseudosolubilizing factor (C18PSF) and pristane pseudosolubilizing factor (PrisPSF) was found. From the partial chemical analysis of the chromatographically purified samples the pseudosolubilizing factors (PSFs) and n-hexadecane emulsifying factor (HEF-C-16) were tentatively identified as glycoprotein and lipoprotein, respectively. Each of the purified PSFs showed molecular weight 14,000 in SDS-PAGE but they had different amino acid compositions. The carbohydrate component of the C16PSF consists of 93% glucose residues. C-12-, C-13-, C-14- and C-16-are the major fatty acids constituting 64% of the total fatty acids of HEF-C-16.