In this study, novel zwitterionic glycosyl modified polyethersulfone (PES) ultrafiltration membranes were prepared via in-situ cross-linking polymerization coupled with phase inversion technique, and the following reactions. The membranes were characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), (HNMR)-H-1 spectrum, and static water contact angles (WCAs) measurements. The modified membranes showed excellent anti-fouling property, and the flux recovery ratio could reach almost 100%. Meanwhile, the blood compatibility of the membranes was measured by protein adsorption, platelet adhesion, activated partial thromboplastin time (APTT), and thrombin time (TT). The results implied that the zwitterionic glycosyl modified PES membranes had good anti-fouling property and blood compatibility. (C) 2014 Elsevier Inc. All rights reserved.