In vitro selection of a photoresponsive peptide aptamer to glutathione-immobilized microbeads

Tada S; Zang QM; Wang W; Kawamoto M; Liu MZ; Iwashita M; Uzawa T; Kiga D; Yamamura M; Ito Y

Journal of Bioscience and Bioengineering, Vol.119, No.2, 137-139, 2015

DOI10.1016/j.jbiosc.2014.06.018 Export Citation

In vitro selection of a photoresponsive peptide aptamer to glutathione-immobilized microbeads

Tada S, Zang QM, Wang W, Kawamoto M, Liu MZ, Iwashita M, Uzawa T, Kiga D, Yamamura M, Ito Y

Photoresponsive peptide aptamer to glutathione-immobilized microbeads was in vitro selected using ribosome display incorporated with tRNA carrying an amino acid coupled with an azobenzene. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.

Keywords:In vitro selection;Photoresponsive aptamer;Peptide aptamer;Photoisomerization;Ribosome display;Misacylated tRNA;Azobenzene