We prepared uncleaved gelatin composed mainly of collagen alpha-, beta-, and gamma-chains. Gelation and melting of uncleaved gelatin occurred rapidly with moderate decrease and increase in temperature (23 degrees C-37 degrees C). The viability of cells encapsulated in the gelatin gel was greater than 96% after 7 d at 23 degrees C. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.