This report emphasizes the use of C-60 as an electron mediator for electrocatalyzed biotransformations. A fullerene, C-60, carboxylic derivative was covalently attached to a cystamine-monolayer-functionalized Au-electrode. The C-60-monolayer revealed a quasi-reversible electrochemical behavior (E-0 = 0.31 V vs SCE) in aqueous electrolyte. The C-60-monolayer (surface density Gamma(C60) = 1.3 x 10(-10) mol cm(-2)) provided electrical communication between the electrode and a soluble glucose oxidase, GOx, with the electron transfer rate constant k(et) = 3 x 10(4) M-1 s(-1). The monolayer-immobilized C-60-mediated bioelectrocatalyzed glucose oxidation in the presence of GOx which was studied by cyclic voltammetry and the rotating disk electrode technique.