In the present technology practiced, hemicelluloses dissolved in the prehydrolysis liquor (PHL) of the kraft-based dissolving pulp production process is mixed with black liquor and incinerated in the kraft mill. In this study, solvent precipitation was used for isolating lignin and hemicelluloses from PHL. The results showed that acetone was a more effective and selective solvent than ethanol to isolate hemicelluloses. Furthermore, the cationization of hemicelluloses with glycidyl-trimethylammonium chloride resulted in cationic hemicelluloses with the charge density of 2.3 meq/g and molecular weight of 6330 g/mol. Based on these results, an integrated dissolving pulp-based biorefining process was proposed that would produce dissolving pulp and cationic hemicellulose as main products.