A bienzyme system aiming at removal of glucose from isomaltooligosaccharide (IMO) was developed in this study. In this bienzymatic system, the first enzyme (glucose oxidase, GOD) catalyzed glucose to generate H2O2 and gluconic acid, while the second one (catalase, CAT) broke down the undesired H2O2 to water and oxygen, which can reduce the peroxide-induced degradation of GOD. For improving the catalytic activity, realizing the recyclability, and simplifying the separation operations of the enzymes, a Pickering emulsion stabilized by GOD- and CAT-containing silica particles was constructed. The optimal reaction temperature and pH were identified as 45 degrees C and 6.0, respectively. Under such conditions, the removal efficiency of glucose could be maintained at ca. 73.57%, even after five cycles in repeated batch operations. It is believed that the proposed process of glucose removal with biocatalytic Pickering emulsion is promising for the purification of IMO on an industrial scale.