The compact geometry and outstanding mechanical properties of ceramic monoliths are valuable features when designing reusable standard affinity chromatography columns. High throughput protein separations are becoming standard industrial practice. Column reuse is an important consideration for the development of separation processes when production rate per unit volume of column is taken into account. Due to the cost and downtime needed to regenerate chromatographic columns, throughput depends on the use of the same column for several adsorptiondesorption cycles without a significant loss in adsorption. An adsorptiondesorption dynamic model was developed and used to estimate the optimum reuse of ceramic monolith columns in affinity chromatography. The model experimental metal chelate affinity chromatography (IMAC) system is a ceramic monolith covered with agarose type D-5 activated with 1.4 butanediol diglycidyl ether as spacer arm, iminodiacetic acid as chelating agent, and Cu2+ as ligand. The effect of performing several adsorption/elution cycles on column performance was evaluated using five monoliths at different flow rates, for a total of five cycles each. The strategy used to interpret experimental results leads to a theoretical model for successive monolith reuses that can be readily used to determine the optimal reuse of monoliths on the basis of maximum production rate per volume of chromatographic columns.