A straightforward method for preparation of carbon nanoparticulate film electrodes is reported. Durable interfaces were prepared by electrophoretic deposition of phenylsulphonate functionalized carbon nanoparticles. The amount of the deposited material and therefore the magnitude of the faradaic current was controlled by tuning the electrophoresis parameters. The prepared electrodes exhibited electrocatalytic effect towards oxidation of thiocholine and showed a linear signal response in a wide concentration range (10.7 - 2000 mu M). The topographies of selected electrodes were investigated by hopping cone-shaped nanoelectrode using a scanning electrochemical microscopy setup in dry conditions. The proposed method of surface examination can be applied to large lateral areas of other nanostructured interfaces. (C) 2014 Elsevier Ltd. All rights reserved.