Collagen is increasingly used as material for medical devices because of its excellent biocompatibility. To achieve long-term durability, the collagen materials are dried and subsequently sterilized. Most common sterilization methods are unsuitable as they cause denaturation. Currently, only ethylenoxide or c irradiation preserves the microscopic and macroscopic collagen structure. However, both methods require high safety standards and include the risk of uncontrolled crosslinking or cleavage of protein chains. Here, supercritical carbon dioxide is used for the sterilization of different collagen devices. Thereby, the influence of pressure, temperature, sterilization aid and dosage, and different operation modes is determined. The method is a promising alternative to existing sterilization techniques.