Botryococcus braunii (B. braunii), a green colonial microalga, is one of the most potentially significant renewable energy sources, because of its high productivity of hydrocarbon oils. In order to maximize energy utilization from this alga, it should be important to recover energy from not only the extracted oil but also the biomass residue after oil-extraction. In this work, oil-extracted residue biomass of an identified strain of B. braunii (BOT-22) with different original hydrocarbon content (HC%: from 28 to 51 wt%) has been characterized as a biofuel feedstock in terms of the elemental composition as well as the pyrolytic behavior by thermogravimetric (TG) analysis and steam pyrolysis test. This paper has revealed that the B. braunii with higher HC%, even biomass residue after oil-extraction, is superior in quality of a biofuel feedstock because of higher content of C, H and volatile matter and lower content of N, O, S, P, ash and fixed carbon as compared to that with lower HC% and other algal biomass. Steam pyrolysis of the oil-extracted residue biomass of the B. braunii at 873-973 K can produce highly carbonized char (similar to 10%-C), gases of C1-C4 (similar to 30%-C) and H-2, and tar (similar to 60%-C) including mainly heavy component such as polycyclic aromatic hydrocarbons. From the analysis of the pyrolyzed products and its higher heating values from 29 to 36 MJ/kg, the residue biomass of B. braunii can be classified as a hydrogen-rich solid fuel comparable to fossil fuel such as a coal rather than other algal and terrestrial biomass. In summary, not only hydrocarbon oils produced from the B. braunii but also its oil-extracted residue biomass should be a superior feedstock for biofuel production. (C) 2014 Elsevier Ltd. All rights reserved.