화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.23, No.2, 205-213, February, 2015
DOI10.1007/s13233-015-3025-1  Export Citation
Effects of surface properties of bacterial poly(3-hydroxybutyrate-co-3-hydroxyvalerate) on adhesion and proliferation of mouse fibroblasts
Victor N. Vasilets1, 2, †, Valentina A. Surguchenko1, 3, Anna S. Ponomareva1, 3, Eugene A. Nemetz1, 4, Victor I. Sevastianov1, 3, Jin Woo Bae5, and Ki Dong Park5, †
  • 1V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs Moscow, Moscow, 123182, Russia, Russian Federation
  • 2V.L. Talrose Institute for Energy Problems of Chemical Physics, Chernogolovka, 142432, Russia, Russian Federation
  • 3Institute of Biomedical Research and Technology, Moscow, 123557, Russia, Russian Federation
  • 4, Russian Federation
  • 5Department of Molecular Science and Technology, Ajou University, 5 Woncheon, Yeongtong, Gyeonggi, 443-749, Korea
E-mail:,
Adhesion and proliferation of NIH/3T3 mouse fibroblasts on the surfaces of bacterial copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and its mixture with poly(ethylene glycol) with differing crystallinity, surface energy, and mean roughness was investigated. The surface mean roughness of all films on both sides, at the air interface and at the glass interface, measured by atomic force microscopy, was higher (from 17.0±1.4 nm to 290.8±7.0 nm) than that of the tissue culture polystyrene control (9.5±0.6 nm). The structure, surface energy, and chemical composition of bacterial films were studied by differential scanning calorimetry, contact angle measurements, and Fourier transform infrared (FTIR) spectroscopy. After 48 h, cell proliferation on all surfaces was significantly less than on the control substrate; however, after 72 and 96 h, cell proliferation was comparable with control on some surfaces with sufficient roughness. Addition of poly(ethylene glycol) resulted in an increase of adhesion and the metabolic activity of the cells, even for relatively smooth surfaces. The complex correlation of cell metabolic activity with surface energy and crystallinity for “rough” (mean roughness >100 nm) and “smooth” (mean roughness < 100 nm) surfaces is discussed.
Keywords:bacterial copolymer;roughness;surface energy;crystallinity;fibroblasts
  1. Volova TG, Shishatskaya EI, Sevastianov VI, Efremov SN, Mogilnaya OM, Biochem. Bioeng. J., 16, 125 (2003)
  2. Volova TG, Sevastianov VI, Shishatskaya EI, Biodegradable Polymers for Medicine. (2006)
  3. Zheng Z, Bei FF, Tian HL, Chen GQ, Biomaterials, 26, 3537 (2005)
  4. Yang M, Zhu S, Chen Y, Chang Z, Chen G, Gong Y, Zhao N, Zhang X, Biomaterials, 25, 1365 (2004)
  5. Qu XH, Wu Q, Zhang KY, Chen GQ, Biomaterials, 27, 3540 (2006)
  6. Noda I, Green PR, Satkowski MM, Schechtman LA, Biomacromolecules, 6(2), 580 (2005)
  7. Chen GQ, Wu Q, Biomaterials, 26, 6565 (2005)
  8. Sundaramurthi D, Krishnan UM, Sethuraman S, J. Biomed. Nanotechnol., 9, 1383 (2013)
  9. Xia LL, Li YS, Zhou Z, Dai Y, Liu HB, Liu HR, Mater. Sci. Eng. C: Mater. Biol. Appl., 33, 3545 (2013)
  10. Wang NN, Zhou Z, Xia LL, Dai Y, Liu HR, Mater. Sci. Eng. C: Mater. Biol. Appl., 33, 2294 (2013)
  11. Masaeli E, Morshed M, Nasr-Esfahani MH, Sadri S, Hilderink J, Apeldoorn A, Blitterswijk CA, Moroni L, PLoS ONE, 8, 57157 (2013)
  12. Lu LX, Zhang XF, Wang YY, Ortiz L, Mao X, Jiang ZL, Xiao ZD, Huang NP, ACS Appl. Mater. Interfaces, 5, 319 (2013)
  13. Wollenweber M, Domaschke H, Hanke T, Boxberger S, Schmack G, Gliesche K, Scharnweber D, Worch H, Tissue Eng., 12, 345 (2006)
  14. Marchessault RH, Bluhm TL, Deslandes Y, Hamer GK, Ortst WJ, Sundararajan PR, Taylor MG, Macromol. Symp., 19, 235 (1988)
  15. Wang YW, Wu Q, Chen J, Chen GQ, Biomaterials, 26, 899 (2005)
  16. Wang YW, Wu Q, Chen GQ, Biomaterials, 25, 669 (2004)
  17. Gao Y, Kong L, Zhang L, Gong Y, Chen G, Zhao N, Eur. Polym. J., 42, 764 (2006)
  18. Rebello H, FDA Requests Recall of All Shelhigh Medical Devices (2007)
  19. Barbucci R, Torricelli P, Fini M, Pasqui D, Favia P, Sardella E, d’Agostino R, Giardino R, Biomaterials, 26, 7596 (2005)
  20. Taguenang JM, Kassu A, Sharma A, J. Colloid Interface Sci., 303(2), 525 (2006)
  21. Wang YW, Wu Q, Chen GQ, Biomaterials, 24, 4621 (2003)
  22. Tesema Y, Raghavan D, Stubbs J, J. Appl. Polym. Sci., 93(5), 2445 (2004)
  23. Mwale F, Wang HT, Nelea V, Luo L, Antoniou J, Wertheimer MR, Biomaterials, 27, 2258 (2006)
  24. Massia SP, Hubbell JA, J. Biol. Chem., 267, 10133 (1992)
  25. Deligianni DD, Katsala ND, Koutsoukos PG, Missirlis YF, Biomaterials, 22, 87 (2001)
  26. Li B, Ma Y, Wang S, Moran PM, Biomaterials, 26, 4956 (2005)
  27. Singhvi R, Stephanopoulos G, Wang DI, Biotechnol. Bioeng., 43(8), 764 (1994)
  28. Chesmel KD, Clark CC, Brighton CT, Black J, J. Biomed. Mater. Res., 29, 1101 (1101)
  29. Hatano K, Inoue H, Kojo T, Matsunaga T, Tsujisawa T, Uchiyama C, Uchida Y, Bone, 25, 439 (1999)
  30. Wan Y, Wang Y, Liu Z, Qu X, Han B, Bei J, Wang S, Biomaterials, 26, 4463 (2005)
  31. Liao H, Andersson AS, Sutherland D, Petronis S, Kasemo B, Thomsen P, Biomaterials, 24, 649 (2003)
  32. Riehle M, Dalby M, Johnstone H, Mackintosh A, Afforssman S, Mater. Sci. Eng. C: Mater. Biol. Appl., 23, 337 (2003)
  33. Keen I, Raggatt LJ, Cool SM, Nurcombe V, Fredericks P, Trau M, Grøndahl L, J. Biomater. Sci. Polym. Ed., 18, 1101 (2007)
  34. Yu BY, Chen PY, Sun YM, Lee YT, Young TH, J. Biomater. Sci. Polym. Ed., 21, 17 (2010)
  35. Kuppan P, Vasanthan KS, Sundaramurthi D, Krishnan UM, Sethuraman S, Biomacromolecules, 12(9), 3156 (2011)
  36. Wang YW, Yang F, Wu Q, Cheng YC, Yu PHF, Chen J, Chen GQ, Biomaterials, 26, 755 (2005)
  37. Gao Y, Kong L, Zhang L, Gong Y, Chen G, Zhao N, Eur. Polym. J., 42, 764 (2006)
  38. Koyama N, Doi Y, Polymer, 38(7), 1589 (1997)
  39. Koyama N, Doi Y, Macromolecules, 29(18), 5843 (1996)
  40. Zhang LL, Xiong CD, Deng XM, Polymer, 37(2), 235 (1996)
  41. Parra DF, Fusaro J, Gaboardi F, Rosa DS, Polym. Degrad. Stab., 91, 1954 (2006)
  42. Hao JY, Deng XM, Polymer, 42(9), 4091 (2001)
  43. Janczuk B, Bialopiotrovicz T, Wojcik W, J. Colloid Interface Sci., 127, 59 (1989)
  44. Zenkiewicz M, J. Achieve. Mater. Manuf. Eng., 24, 137 (2007)
  45. Wang YW, Yang F, Wua Q, Chengc YC, Yuc PHF, Chena J, Chena GQ, Biomaterials, 26, 755 (2005)
  46. Boyan BD, Hummert TW, Dean DD, Schwartz Z, Biomaterials, 17, 137 (1996)
  47. Hanein D, Geiger B, Addadi L, Science, 263(5152), 1413 (1994)
  48. Faghihi S, Batene MR, Azari F, Szpunar JA, Vali H, Tabrizian M, Mater. Sci. Forum, 705, 495 (2005)
  49. Chan RTH, Garvey CJ, Marc H, Russell RA, Holden PJ, Foster LJR, Int. J. Polym. Sci., 2011, 1549 (2011)
  50. Farrugia BL, Brooke L, Keddie DJ, Daniel J, George GA, Graeme A, Lynam EC, Brook MA, Upton Z, Dargaville TR, J. Biomed. Mater. Res. A, 100, 1919 (2012)
  51. Zhang F, Huang Y, Li XD, Zhao SF, Int. J. Oral Maxillofac. Implants, 26, 1183 (2011)
  52. Park H, Lee JW, Park KE, Park WH, Lee KY, Colloids Surf. B, 77, 90 (2010)