Ligand immobilization on polydiacetylene-coated and surface enhanced Raman scattering-encoded beads for label-free detection

Hyung-Mo Kim; Yoo-Lee Kang; Woo-Jae Chung; San Kyeong; Sinyoung Jeong; Homan Kang; Cheolhwan Jeong; Won-Yeop Rho; Dong-Hyuk Kim; Dae Hong Jeong; Yoon-Sik Lee; Bong-Hyun Jun

Journal of Industrial and Engineering Chemistry, Vol.21, 158-162, January, 2015

DOI10.1016/j.jiec.2014.06.010 Export Citation

Ligand immobilization on polydiacetylene-coated and surface enhanced Raman scattering-encoded beads for label-free detection

Hyung-Mo Kim¹, Yoo-Lee Kang¹, Woo-Jae Chung², San Kyeong³, Sinyoung Jeong⁴, Homan Kang⁵, Cheolhwan Jeong³, Won-Yeop Rho¹, Dong-Hyuk Kim¹, Dae Hong Jeong⁴, Yoon-Sik Lee^{3, 5}, and Bong-Hyun Jun^{1, †}

¹Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea
²Department of Genetic Engineering, College of Biotechnology & Bioengineering, Sungkyunkwan University, Suwon, Gyeonggi-do 440-746, Republic of Korea
³School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Republic of Korea
⁴Department of Chemistry Education, Seoul National University, Seoul 151-747, Republic of Korea
⁵Nano Systems Institute and Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul 151-742, Republic of Korea

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A better understanding of protein.protein interactions can be obtained from multiplex protein detection technologies, and spectrally encoded beads can provide fast and efficient means for this type of detection methods. However, high-throughput detection is challenging due to the requirement of using labeled secondary proteins to detect protein binding events.We have previously reported that polydiacetylenecoated surface-enhanced Raman scattering-encoded beads (PDA-SERS beads) can provide an enhanced encoding capacity owing to their SERS properties as well as their potential for label-free detection from the PDA layer. In this study, we introduced ligands to the PDA-SERS beads by using methods for making free-floating vesicles and planar solid substrates, which enabled the detection of target proteins by PDA fluorescence in a PDA.SERS beads system. By using PDA.SERS beads immobilized with biotin, the fluorescence intensities of biotin-conjugated PDA.SERS beads were increased with an increase in the concentration of streptavidin. And, we could detect 2×10^(-8)M of streptavidin by measuring the fluorescence intensity without the requirement of an additional labeling step.

Keywords:Polydiacetylene (PDA);Micro-sized bead;Label-free detection;Protein detection

[References]

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Jun BH, Kang H, Lee YS, Jeong DH, Molecules, 17, 2474 (2012)
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Balboni I, Chan SM, Kattah M, Tenenbaum JD, Butte AJ, Utz PJ, Annu. Rev. Immunol., 24, 391 (2006)
Jun BH, Hwang DW, Jung HS, Jang J, Kim H, Kang H, Kang T, Kyeong S, Lee H, Jeong DH, Kang KW, Youn H, Lee DS, Lee YS, Adv. Funct. Mater., 22(9), 1843 (2012)
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Claudio V, Dahlin AB, Antosiewicz TJ, J. Phys. Chem. C, 118, 6980 (2014)
Charych DH, Nagy JO, Spevak W, Bednarski MD, Science, 261, 585 (1993)
Okada S, Peng S, Spevak W, Charych D, Acc. Chem. Res., 31, 229 (1998)
Rangin M, Basu A, J. Am. Chem. Soc., 126(16), 5038 (2004)
Song J, Cisar JS, Bertozzi CR, J. Am. Chem. Soc., 126(27), 8459 (2004)
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Pan JJ, Charych D, Langmuir, 13(6), 1365 (1997)
Jaworski J, Yokoyama K, Zueger C, Chung WJ, Lee SW, Majumdar A, Langmuir, 27(6), 3180 (2011)
Jun BH, Kim JH, Park H, Kim JS, Yu KN, Lee SM, Choi H, Kwak SY, Kim YK, Jeong DH, Cho MH, Lee YS, J. Comb. Chem., 9, 237 (2007)
Jun BH, Kim G, Noh MS, Kang H, Kim YK, Cho MH, Jeong DH, Lee YS, Nanomedicine, 6, 1463 (2011)
Jun BH, Baek J, Kang H, Park YJ, Jeong DH, Lee YS, J. Colloid Interface Sci., 355(1), 29 (2011)
Baughman R, Chance R, J. Polym. Sci., Polym. Phys. Ed., 14, 2037 (1976)
Olmsted III J, Strand M, J. Phys. Chem., 87, 4790 (1983)
Carpick RW, Mayer TM, Sasaki DY, Burns AR, Langmuir, 16(10), 4639 (2000)
Carpick RW, Sasaki DY, Burns AR, Langmuir, 16(3), 1270 (2000)
Sheth SR, Leckband DE, Langmuir, 13(21), 5652 (1997)
Horejsh D, Martini F, Poccia F, Ippolito G, Di Caro A, Capobianchi MR, Nucleic Acids Res., 33, 13 (2005)
Jung YK, Park HG, Kim JM, Biosens. Bioelectron., 21, 1536 (2006)

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[3] Kevin B, De Stefaan SC, Marc L, Chris R, Rudi P, Joseph D, Mod. Drug Discovery, 6, 28 (2003)

[4] Finkel NH, Lou X, Wang C, He L, Anal. Chem., 76, 352 (2004)

[5] Borisov SM, Wolfbeis OS, Chem. Rev., 108(2), 423 (2008)

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[9] Derveaux S, Stubbe B, Braeckmans K, Roelant C, Sato K, Demeester J, De Smedt S, Anal. Bioanal. Chem., 391, 2453 (2008)

[10] Claudio V, Dahlin AB, Antosiewicz TJ, J. Phys. Chem. C, 118, 6980 (2014)

[11] Charych DH, Nagy JO, Spevak W, Bednarski MD, Science, 261, 585 (1993)

[12] Okada S, Peng S, Spevak W, Charych D, Acc. Chem. Res., 31, 229 (1998)

[13] Rangin M, Basu A, J. Am. Chem. Soc., 126(16), 5038 (2004)

[14] Song J, Cisar JS, Bertozzi CR, J. Am. Chem. Soc., 126(27), 8459 (2004)

[15] Schwenk JM, Lindberg J, Sundberg M, Uhlen M, Nilsson P, Mol. Cell. Proteomics, 6, 125 (2007)

[16] Pan JJ, Charych D, Langmuir, 13(6), 1365 (1997)

[17] Jaworski J, Yokoyama K, Zueger C, Chung WJ, Lee SW, Majumdar A, Langmuir, 27(6), 3180 (2011)

[18] Jun BH, Kim JH, Park H, Kim JS, Yu KN, Lee SM, Choi H, Kwak SY, Kim YK, Jeong DH, Cho MH, Lee YS, J. Comb. Chem., 9, 237 (2007)

[19] Jun BH, Kim G, Noh MS, Kang H, Kim YK, Cho MH, Jeong DH, Lee YS, Nanomedicine, 6, 1463 (2011)

[20] Jun BH, Baek J, Kang H, Park YJ, Jeong DH, Lee YS, J. Colloid Interface Sci., 355(1), 29 (2011)

[21] Baughman R, Chance R, J. Polym. Sci., Polym. Phys. Ed., 14, 2037 (1976)

[22] Olmsted III J, Strand M, J. Phys. Chem., 87, 4790 (1983)

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[24] Carpick RW, Sasaki DY, Burns AR, Langmuir, 16(3), 1270 (2000)

[25] Sheth SR, Leckband DE, Langmuir, 13(21), 5652 (1997)

[26] Horejsh D, Martini F, Poccia F, Ippolito G, Di Caro A, Capobianchi MR, Nucleic Acids Res., 33, 13 (2005)

[27] Jung YK, Park HG, Kim JM, Biosens. Bioelectron., 21, 1536 (2006)