화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.32, No.3, 413-423, March, 2015
DOI10.1007/s11814-014-0235-8  Export Citation
Improvement of cassava stem hydrolysis by two-stage chemical pretreatment for high yield cellulosic ethanol production
Bunpot Klinpratoom1, 2, Anissara Ontanee1, and Chalerm Ruangviriyachai1, 2, †
  • 1Department of Chemistry and Center of Excellence for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
  • 2National Research University Project of Thailand, Biofuel Cluster, Khon Kaen University, Khon Kaen 40002, Thailand
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We used sodium chlorite followed by sodium hydroxide as a two-stage pretreatment of cassava stem for removal of lignin and hemicellulose to obtain a substrate with high cellulose content prior to hydrolysis. Response surface methodology was applied to determine the optimum hydrolysis conditions of two-stage pretreated cassava stem. After pretreatment, the cellulose content of cassava stem increased from 42.10% to 86.45%, concomitant with decreases in lignin (87.59%) and hemicellulose (78.18%) content. Acid hydrolysis of two-stage pretreated cassava stem under optimum conditions allowed obtaining a hydrolyzate rich in reducing sugar, with a yields up to 67.37%. Conversely, inhibitors were detected at very low concentrations. The fermentation of the hydrolyzate resulted in an ethanol yield of 22.58 g/100 g substrate corresponding to a theoretical ethanol yield of 84.41%. The results demonstrate that two-stage pretreatment is effective for improving cellulose hydrolyzability, resulting in high fermentable sugar and low fermentation inhibitor concentrations.
Keywords:Cassava Stem;Two-stage Pretreatment;Response Surface Methodology;Reducing Sugar;Cellulosic Ethanol
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