Sequential optimization strategy for hyaluronic acid extraction from eggshell and its partial characterization

Mehdi Khanmohammadi; Ali Baradar Khoshfetrat; Shahla Eskandarnezhad; Najimeh Feyze Sani; Sirus Ebrahimi

Journal of Industrial and Engineering Chemistry, Vol.20, No.6, 4371-4376, November, 2014

DOI10.1016/j.jiec.2014.02.001 Export Citation

Sequential optimization strategy for hyaluronic acid extraction from eggshell and its partial characterization

Mehdi Khanmohammadi^{1, 2}, Ali Baradar Khoshfetrat^{1, 2, †}, Shahla Eskandarnezhad^{1, 2}, Najimeh Feyze Sani^{1, 2}, and Sirus Ebrahimi¹

¹Department of Chemical Engineering, Sahand University of Technology, Tabriz 51335-1996 Iran
²Stem Cell and Tissue Engineering Research Laboratory, Sahand University of Technology, Tabriz 51335-1996, Iran

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The optimization of hyaluronic acid (HA) extraction from eggshell was studied by using a sequential experimentation approach. Using the results of factorial experiments, optimization experiments were carried out by response surface methodology. The experimental data as well as the validation testsshowed the optimum performance when the extraction pH and time were bounded in the ranges of 3.4-3.5 and 3-3.4 days, respectively, at 9 °C. The purified HAwas characterized by FTIR spectrum, harmonic mean size and average molecularweight. The study depicted the potential of eggshell as an alternative source for HA extraction to use in industry and medicine.

Keywords:Characterization;Eggshell;Experimental design;Hyaluronic acid extraction;Sequential optimization strategy

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[1] Kogan G, Soltes L, Stern R, Gemeiner P, Biotechnol. Lett., 29(1), 17 (2007)

[2] Lee HG, Cowman MK, Anal. Biochem., 219, 278 (1994)

[3] Cowman MK, Matsuoka S, Carbohydr. Res., 340, 791 (2005)

[4] Knudson CB, Knudson W, Faseb J., 7, 1233 (1993)

[5] Goa KL, Benfield P, Drugs, 47, 536 (1994)

[6] Balazs EA, Leshchiner A, Larsen N, Band F, US Patent 5 099 013, 1992.

[7] A. Prescott, US Patent 6 660 853, 2003.

[8] Long FD, Adams RG, Devore DP, US Patent 0 180 851, 2004.

[9] Patil KP, Patil DK, Chaudhari BL, Chincholkar SB, J. Biosci. Bioeng., 111, 286 (2010)

[10] Rangaswam V, Jain D, Biotechnol. Lett., 30, 493 (2010)

[11] Coutts JA, Wilson GC, Optimum Egg Quality: A Practical Approach, 5M Publishing, Sheffield, 2006.

[12] Bitter T, Muir HM, Anal. Biochem., 4, 330 (1962)

[13] Gura E, Hucker M, Muller G, Polym. Degrad. Stabil., 59, 297 (1998)

[14] Berbaum M, US Patent 7 002 007, 2006.

[15] Tokita Y, Okamoto A, Eur. Polym. J., 32, 1011 (1996)

[16] Tommeraas K, Melander C, Biomacromolecules, 9(6), 1535 (2008)

[17] Maleki A, Kjoniksen AL, Nystrom B, Macromol. Symp., 274, 131 (2008)

[18] Mehta DP, Shodhan K, Modi RI, Ghosh PK, Curr. Sci., 92, 209 (2007)

[19] Aliakbarian B, De Faveri D, Converti A, Perego P, Biochem. Eng. J., 42, 34 (2008)

[20] Myers RH, Montgomery DC, Anderson-Cook CM, Response surface methodology: process and product optimization using designed experiments, 3rd ed., Wiley, New York, 2009.