Removal rates of hydrogen sulfide were investigated to known effects of several light sources with external and internal irradiation on the desulfurization using C. thiosulfatophilum. In the case of internal illumination system, optical-fiber photobioreactor was applied to increase the light availability. Furthermore, sunlight was used as the main light energy in the daytime and metal-halide lamp was applied as an additional light energy at night. Light energy of 99% was saved by the application of the LED's array in comparison with the incandescent light source. H2S removal rates at 5,000 lux in a 4-L photobioreactor were shown as 0.040, 0.138, 0.136, and 0.134 (μmol H2S/min)/(mg protein/L), respectively, in the following order of light sources, when several light sources such as fluorescent, energy-saving, incandescent, halogen lamp, and filtered light at 460 nm were applied. Removal rate per unit cell concentration with the internal light diffused optical-fibers increased about 1 six times as much as that with the external light sources. Removal rate per unit cell concentration, using sunlight in the daytime and a metal-halide lamp at night, was 0.41 less than 0.869 (μmol H2S/min)/(mg protein/L) using a 400 W metal-halide lamp day and night, since the automatic sunlight collection system can transmit the light intensity as only 10% of that with a metal-halide lamp.