Highly ordered N-doped titania mesoporous inorganic-organic hybrid thin films were prepared according to a refined recipe by an evaporative induced self-assembly route using Pluronic P123 as the template. Films were characterized by a grazing incident small angle X-ray scattering, atomic force microscopy, X-ray photoelectron spectroscopy, Raman spectroscopy and X-ray diffraction. Visible light induced bactericidal activity was performed on Gram-positive bacteria (Bacillus amyloliquifacience (NCIM 2829)). Bacterial cell death was confirmed by cell viability assay and inhibition of bacterial growth against reactive oxygen species induction is reported. (C) 2012 Elsevier B.V. All rights reserved.