Sandblasted large grit and acid treatment (SLA) is the most common approach to alter the surface topography and increase the surface area of an implant. In this study, plasma polymerized SLA-treated titanium(Ti) surfaces were prepared by the deposition of a polymeric thin film containing COOH, NH2 and OH groups through the plasma polymerization of acrylic acid, allyl amine and allyl alcohol, respectively. The Ti surfaces were characterized by field emission scanning electron microscopy, attenuated total reflectance-Fourier transform infrared and contact angle measurements. The proliferation and differentiation of MC3T3-E1 cell cultured on the Ti, SLA and plasma polymerized SLA/Ti surfaces were examined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and alkaline phosphatase assay. The cell proliferation study revealed significantly enhanced cell viability on the COOH/SLA and NH2/SLA surfaces compared to the other surfaces. (C) 2012 Elsevier B. V. All rights reserved.