An experimental investigation of the electrokinetic transport of negative and positive polystyrene beads in alcohol-water mixtures is described. The electrophoretic phenomenological coefficient has been measured, and a nonlinear dependence on the alcohol concentration has been found. The liquid properties (density, viscosity, and dielectric constant) and the zeta-potential have been shown to play a significant role in the internal structure of the electrophoretic coefficient (l(21)) for the negative polystyrene/(ethanol or 1-propanol) -water mixture. This behavior is quite different for positive polystyrene. A typical hydrophobic character of the positive polystyrene contrast with the double hydrophobic-hydrophilic character of the negative one.