Plasmonic nanoprobes functionalized with morpholino oligos have been developed for genotyping of single-nucleotide polymorphisms (SNPs) based on calorimetric signals that could be easily visualized. The assay was conducted with a standard thermal cycler that would be available in any molecular diagnostic laboratory, facilitating assay deployment and allowing for cost-effective genetic testing with rapid turnaround time. Specifically, a warfarin sensitivity testing kit gauging three SNPs has been created and validated with 249 human genomic DNA samples in four independent institutes, demonstrating the robustness of our new platform. (C) 2014 Elsevier Ltd. All rights reserved.