The use of comprehensive two-dimensional gas chromatography (GCxGC) is becoming more popular for complex sample analysis and the optimization of this technique is paramount for accurate qualitative and quantitative reporting. In this work, the effect of detector data acquisition rate (or detector sampling speed) for GCxGC is investigated and summarized. Effects on chromatographic signal such as peak area, height, and width, as well as analytical figures of merit such as resolution and limit of detection, are investigated at four data acquisition rates using a multi-component test mixture. Adjusting the data acquisition rate of the detector has a profound effect on peak areas, heights, and peak widths. In addition, the increase in detected signal and decreased peak widths led to improved chromatographic resolution and limits of detection (LOD) under optimized conditions.