A novel metal-carboxylate system metal-organic frameworks ([Zn-2(cam)(2)dabco] (Zn-CD)) membrane was synthesized on porous ZnO support and used for gas separation. XRD and SEM were used to characterize the structure, composition, and morphology of the membrane. The results proved that the uniform Zn-CD seeding layer and continuous Zn-CD membrane were effectively achieved by reactive seeding method. Through optimizing the preparation process, the integrated Zn-CD membrane was obtained. The single and binary gas permeations of the Zn-CD membranes were systematically investigated. The separation factors of H-2/N-2 and H-2/CH4 gas mixture reach to 19.1 and 14.5, respectively, indicating that the Zn-CD membranes are defect free and have great potential applications for hydrogen separation. (C) 2013 Elsevier B.V. All rights reserved.