Chiral separation of enantiomers of amlodipine, one of the most commonly prescribed antihypertensive drugs, was examined using the hollow fiber supported liquid membrane (HFSLM) extraction technique. The influence of temperature on enantioseparation of (R,S)-amlodipine via a HFSLM containing the chiral selector O,O'-dibenzoyl-(2S,3S)-tartaric acid ((+)-DBTA) was systematically investigated. The parameters affecting the mass transfer such as distribution ratio and flux were determined at different temperatures ranging from 278.15 K to 313.15 K. The thermodynamic parameters, Delta H and Delta G, were determined, and an interesting relationship with stoichiometric value was found: higher temperatures lead to an increase in distribution ratio but a decrease in enantioselectivity. The activation energy (E-a) of the (S)-amlodipine extraction reaction was 71.10 kJ/mol. The chemical reaction between (S)-amlodipine and (+)-DBTA is the mass transfer-controlling step for the enantioseparation of (S)-amlodipine by a hollow fiber supported liquid membrane system. (C) 2012 Elsevier B.V. All rights reserved.