The present study focused on the recovery of collagen and its hydrolysate from a homogenate obtained by enzymatic hydrolysis of bovine hide. The influence of Bat enzyme, protease and pepsin on the hydrolysis of hide material is investigated. Aqueous two-phase systems (ATPS) having a composition of 14% polyethylene glycol (PEG) 600 and 15% potassium phosphate was loaded with 20% w/w of collagen homogenate. Such loaded systems yielded a two phase system, a top phase (97% recovery yield of collagen) and a bottom phase (94% recovery yield of pepsin enzyme). The study indicated that it is possible to selectively obtain collagen and its hydrolysed product in one phase and the enzyme used for the hydrolysis in another phase in ATPS partition. (c) 2012 Elsevier B.V. All rights reserved.