An integrated, clean, facile and ecologically friendly approach of biodiesel production from Eruca Sativa Gars (ESG) vegetable oil focused on lab scaling up was reported in this study. Transesterification of ESG oil was heterogeneously catalyzed by Cs(2.5)H(0.5)PW(12)O(40) heteropolyacid salt. The properties of biodiesel from ESG were comparable to conventional diesel fuel and comply with the US Standard for Biodiesel (ASTM 6751). Using ESG biodiesel instead of conventional diesel fuel reduces emissions. The results illustrate that the Cs(2.5)H(0.5)PW(12)O(40) is an environmentally benign solid acid catalyst and ESG biodiesel is a kind of nontoxic and biodegradable renewable alternative fuel. (C) 2008 Published by Elsevier Ltd.