Surface exclusion chromatography is particularly suitable to determine the structure of highly dilute solutions, where more common methods such as light scattering are inefficient and other chromatographic techniques cannot be employed due to strong adsorption of the solutes on the stationary phases. This process was simulated using two kinetic models (localized and mobile adsorption) to randomly deposit solute particles on the adsorbent plates of a chromatographic column. The influence of the relative size and concentration of two different adsorbing species on the degree of coverage of the successive plates of the column was investigated, and chromatograms derived from simulation were compared to those for the chromatographic separation by mutual surface exclusion of unimers and micelles in solutions of a diblock copolymer.