Environmentally Safe Polyhydroxybutyrate Synthesis by Alcaligenes eutrophus in Pressurized Fermentor

Kyeo-Keun Kim; Kyoung Shin

Journal of Industrial and Engineering Chemistry, Vol.2, No.1, 18-25, June, 1996

Dept. of Environ. Eng., Chongju Univ., Chongju 360-764, Korea
¹Dept. of Chem. Eng., New Mexico State Univ., Las Cruces, NM, USA

A new high pressure fermentation system was employed for high density cultivation of Alcaligenes eutrophus (ATCC 17699) in order to improve the productivity of Poly-β-hydroxybutyrate(PHB). The effects of pressure and temperature on microbial growth and metabolism was examined. The maximum PHB yield was approximately 62% of the cellular dry weight when the cells were grown on fructose in a high pressure fermentor system at a controlled oxygen composition.

[References]

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Kawaguchi Y, Doi Y, Macromolecules, 25, 2324 (1992)
Doi Y, Kawaguchi Y, Nakamura Y, Kunioka M, Appl. Environ. Microbiol., 55, 2932 (1989)
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Doi Y, Kunioka M, Nakamura Y, Soga K, Macromolecules, 19, 2860 (1986)
Doi Y, Segawa A, Kunioka M, Polym. Commun., 30, 169 (1989)
Kunioka M, Kawaguchi Y, Doi Y, Appl. Microbiol. Biotechnol., 30, 569 (1989)
Linko S, Vaheri H, Seppala J, Enzyme Microb. Technol., 15, 401 (1993)
Hustede E, Steinbuchel A, Schlegel HG, Appl. Microbiol. Biotechnol., 39, 87 (1993)
Gottleib SF, Ann. Rev. Microbiol., 37, 42 (1971)
Prausting H, Hazenberg W, Witholt B, Enzyme Microb. Technol., 13, 770 (1993)
Loung JHT, Biotechnol. Bioeng., 29, 242 (1987)
Schlegel HG, Lafferty RM, Adv. Biochem. Eng., 1, 143 (1971)
Steinbuchel A, Schlegel HG, Appl. Microbiol., 31, 168 (1989)

[1] Fernandez-Castillo R, Rodriguez-Valera F, Gonzalez-Ramos J, Ruiz-Berraquero F, Appl. Environ. Microbiol., 51, 214 (1986)

[2] Doi Y, Tamaki A, Kunioka M, Soga K, Appl. Microbiol. Biotechnol., 28, 330 (1988)

[3] Kunioka M, Nakamura Y, Doi Y, Polym. Commun., 29, 174 (1988)

[4] Doi Y, Tamaki A, Nakamura Y, Soga K, Macromolecules, 21, 2988 (1988)

[5] Kim K, Ph.D. Dissertation, New Mexico State Univ., Las Cruces, U.S.A. (1994)

[6] Kawaguchi Y, Doi Y, Macromolecules, 25, 2324 (1992)

[7] Doi Y, Kawaguchi Y, Nakamura Y, Kunioka M, Appl. Environ. Microbiol., 55, 2932 (1989)

[8] Oeding V, Schlegel HG, Biochem. J., 134, 239 (1973)

[9] Repaske R, Repaske AC, Appl. Environ. Microbiol., 32, 585 (1976)

[10] Standard Methods for Examination of Water and Wastewater, 16th ed., American Health Association, Washington, D.C. (1985)

[11] Schlegel HG, Laffery R, Krauss I, Arch. Microbiol., 71, 283 (1970)

[12] Williamson DH, Wilkinson JF, J. Gen. Microbiol., 19, 198 (1958)

[13] Doi Y, Segawa A, Kunioka M, Int. J. Biol. Macromol., 12, 106 (1990)

[14] Doi Y, Tamaki A, Kunioka M, Soga K, J. Chem. Soc.-Chem. Commun., 1635 (1987)

[15] Doi Y, Kunioka M, Nakamura Y, Soga K, Macromolecules, 19, 2860 (1986)

[16] Doi Y, Segawa A, Kunioka M, Polym. Commun., 30, 169 (1989)

[17] Kunioka M, Kawaguchi Y, Doi Y, Appl. Microbiol. Biotechnol., 30, 569 (1989)

[18] Linko S, Vaheri H, Seppala J, Enzyme Microb. Technol., 15, 401 (1993)

[19] Hustede E, Steinbuchel A, Schlegel HG, Appl. Microbiol. Biotechnol., 39, 87 (1993)

[20] Gottleib SF, Ann. Rev. Microbiol., 37, 42 (1971)

[21] Prausting H, Hazenberg W, Witholt B, Enzyme Microb. Technol., 13, 770 (1993)

[22] Loung JHT, Biotechnol. Bioeng., 29, 242 (1987)

[23] Schlegel HG, Lafferty RM, Adv. Biochem. Eng., 1, 143 (1971)

[24] Steinbuchel A, Schlegel HG, Appl. Microbiol., 31, 168 (1989)