A new method has been developed to determine the activation energy for hydrogen desorption from steels by means of thermal desorption spectrometry (TDS). This method directly fits the Kissinger's reaction kinetic formula dX/dt=A(1-X)exp(-E-d/RT) to experimentally measured thermal desorption spectrum and best fit yields the activation energy (E-d) and the value of constant A. It has been proven that this new method is applicable to precise measurement of the activation energy for hydrogen desorption from incoherent TiC particle, coherent TiC precipitate, grain boundary and dislocation in 0.05C-0.20Ti-2.0Ni and 0.42C-0.30Ti steels.