Treatment of 1,4-dioxane solution by pulsed discharge plasma in air with water droplets spray was investigated for the first time. Positive pulse voltage was supplied to the discharge electrodes by pulsed power generator with a pulse repetition rate of 100 pps. Peak voltage and peak current were 34.4 kV and 168 A, respectively, and discharge power was 17W. 1,4-Dioxane in the solution decreased with discharge treatment time and was not detected after 7-h of treatment. 1,4-Dioxane concentration also decreased without the discharge plasma treatment, but at a lower rate of decrease rate. The results suggested the potential of pulsed discharge plasma in air with water droplets spray for practical use in treatment of 1,4-dioxane.