The three crude extracts including Oryza sativa (bran) from supercritical carbon dioxide fluid (scCO(2)) process which gave the highest unsaturated fatty acid contents and biological activities including the antioxidative, tyrosinase inhibition, stimulation index on human normal skin fibroblast were selected from ten edible plants to prepare the semi-purified fractions. Fraction No. 3 of the O. sativa bran crude extract gave the highest content of unsaturated fatty acids and 5 alpha-reductase (type 1) inhibition activity (5AR). Its linoleic acid (LN) and total unsaturated fatty acid (TUC) contents were significantly positive and linear correlated to 5AR on DU-145 cell line (at r of 1.00, p < 0.01). Its total phenolic contents and all biological activities also showed positive correlations to 5AR with r > 0.9 (p < 0.05). This study has demonstrated the potential of fraction No. 3 fractionated from the O. sativa bran crude extract prepared by scCO(2) to be developed as anti-androgenic alopecia products. (C) 2011 Elsevier B.V. All rights reserved.