Squalene, an intermediate in the biosynthesis of cholesterol, has a 24-carbon backbone with six methyl groups and six isolated double bonds. Capillary flow techniques have been used to determine its translational diffusion constant, D, at room temperature in squalane, n-C-16, and three n-C-8-squalane mixtures. The D values have a weaker dependence on viscosity, eta, than predicted by the Stokes-Einstein relation, D = k(B)T/(6 pi eta r). A fit to the modified relation, D/T = A(SE)/eta(P), gives p = 0.820 +/- 0.028; p = 1 for the Stokes-Einstein limit. The translational motion of squalene appears to be much like that of n-alkane solutes with comparable chain lengths; their D values show similar deviations from the Stokes-Einstein model. The n-alkane with the same carbon chain length as squalene, n-C-24, has a near-equal p value of 0.844 +/- 0.018 in n-alkane solvents. The values of the hydrodynamic radius, r, for n-C-24, squalene, and other n-alkane solutes decrease as the viscosity increases and have a common dependence on the van der Waals volumes of the solute and solvent. The possibility of studying squalene in lipid droplets and membranes is discussed.