This study was focused on the possibility to inactivate main food pathogens, their spores and biofilms on the surface of packaging material polyolefine by Na-chlorophyllin (Na-ChI)-based photosensitization and to compare efficiency of this treatment with conventional antimicrobials. Data indicate that Bacillus cereus and Listeria monocytogenes were effectively inactivated (7 log) by Na-ChI (7.5 x 10(-7) M)-based photosensitization in vitro and on the surface of packaging. Meanwhile to achieve adequate inactivation of thermo-resistant strains, spores or biofilms the higher Na-ChI concentration and longer illumination times had to be used. Comparison of different surface decontamination treatments reveal that photosensitization is much more effective against B. cereus and L. monocytogenes attached on the surface than washing with water or 200 ppm Na-hypochlorite. Our data support the idea that photosensitization may serve in the future for the development of human and environment friendly, non-thermal surface decontamination technique. (C) 2011 Elsevier Ltd. All rights reserved.