Self-Assembly of Rigid Polypeptides

Cheon JB; Jeong YI; Cho CS

Korea Polymer Journal, Vol.6, No.1, 34-40, March, 1998

the amphiphilic block copolymers, consisting of polypeptides [poly(γ-benzyl L-glutamate) or poly(L-ieucinel)]as the hydrophobic component and polyether [or poly(N-isopropylacrylamide) (PNIPAAm)] as the hydrophilic one were prepared by the ring-opening polymerization of the corresponding N-carboxyanhydrides, initiated by the amino-terminated poly(ethylene oxide) (PEO) (or PNIPAAm). These amphiphilic copolymers in water can self-assemble to form stable micelles that are presumed to compose of the hydrophilic shell and the rigid rod-like and hydrophobic core. The micellar properties such as CMC, micelle size, and micelle shape were studied by fluorescent probe techniques, dynamic light scattering, and transmission electron microscopy. respectivwly From application of self-assembled micelles it was found that release characteristics of the hydrophobic drugs (clonazepam or indomethacin) loaded into the core part of these micelles was dependent on the drug loading contents and chain length of the hydrophobic part of the copolymers. The higher the hydrophobic portion in the block copolymer, the smaller the CMC of the copolymer. For the polymeric micelles with PNIPAAm outershell, their CMCs are less affected by temperature than the other polymeric micelles. this property strongly affects the behavior of the drug release from polymeric micelle with PNIPAAm outershell with temperature.

[References]

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Kunitak T, Angew. Chem.-Int. Edit., 31, 709 (1992)
Zhong XF, Varsheny SK, Eisenberg A, Macromolecules, 25, 7160 (1992)
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Nagarajan R, Ganesh K, J. Phys. Chem., 90, 5843 (1989)
Edwards CJC, Richards RW, Stepto RFT, Polymer, 27, 643 (1987)
Ahmad N, Kaleem M, Noor S, Colloid Polym. Sci., 261, 898 (1983)
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Price C, Chan EKM, Hudd AL, Stubbersfield RB, Polym. Commun., 27, 196 (1986)
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Izzo D, Marques CM, Macromolecules, 26, 7189 (1993)
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Robinson C, Mol. Cryst. Liq. Cryst., 1, 467 (1966)
Cho CS, Choen JB, Jeong YI, Kim IS, Kim SH, Akaike T, Macromol. Rapid Commun., 18, 361 (1997)
Jeong YI, Cheon JB, Kim SH, Nah JW, Lee YM, Sung YK, Akaike T, Cho CS, J. Control. Release, 51, 169 (1998)
Cheon JB, Jeong YI, Cho CS, Polymer, published

[Referenced By]

[1] Ahlers M, Muller W, Reicher A, Ringsdorf H, Venzmer J, Angew. Chem.-Int. Edit., 29, 1269 (1990)

[2] Whitesides GM, Mathias JP, Seto CT, Science, 254, 1312 (1991)

[3] Ringsdorf H, Schlarb B, Venzmer J, Angew. Chem.-Int. Edit., 27, 113 (1988)

[4] Debye P, Anacker EW, J. Phys. Colloid Chem., 55, 644 (1951)

[5] Kunitak T, Angew. Chem.-Int. Edit., 31, 709 (1992)

[6] Zhong XF, Varsheny SK, Eisenberg A, Macromolecules, 25, 7160 (1992)

[7] Whitmore MD, Noolandi J, Macromolecules, 18, 657 (1985)

[8] Nagarajan R, Ganesh K, J. Phys. Chem., 90, 5843 (1989)

[9] Edwards CJC, Richards RW, Stepto RFT, Polymer, 27, 643 (1987)

[10] Ahmad N, Kaleem M, Noor S, Colloid Polym. Sci., 261, 898 (1983)

[11] Dondos A, Rempp P, Benoit H, Polymer, 16, 698 (1975)

[12] Mandema W, Emeiss CA, Zeldenrust H, Macromol. Chem., 180, 1521 (1979)

[13] Price C, Pure Appl. Chem., 55, 1561 (1983)

[14] Kalyanasundaram K, Thomas JK, J. Am. Chem. Soc., 30, 2039 (1977)

[15] Price C, Chan EKM, Hudd AL, Stubbersfield RB, Polym. Commun., 27, 196 (1986)

[16] Wihelm M, Zhao CL, Wang Y, Xu R, Winnik MA, Mura JL, Riess G, Croucher MD, Macromolecules, 24, 1033 (1991)

[17] Izzo D, Marques CM, Macromolecules, 26, 7189 (1993)

[18] Gao Z, Eisenberg A, Macromolecules, 26, 7353 (1993)

[19] Kataoka K, Kwon GS, Yokohama M, Okano T, Sakurai Y, J. Control. Release, 24, 119 (1993)

[20] Higuchi T, J. Pharm. Sci., 52, 1145 (1963)

[21] Rhine W, Hsieh D, Langer R, J. Pharm. Sci., 69, 265 (1980)

[22] Doty P, J. Am. Chem. Soc., 78, 947 (1956)

[23] Robinson C, Mol. Cryst. Liq. Cryst., 1, 467 (1966)

[24] Cho CS, Choen JB, Jeong YI, Kim IS, Kim SH, Akaike T, Macromol. Rapid Commun., 18, 361 (1997)

[25] Jeong YI, Cheon JB, Kim SH, Nah JW, Lee YM, Sung YK, Akaike T, Cho CS, J. Control. Release, 51, 169 (1998)

[26] Cheon JB, Jeong YI, Cho CS, Polymer, published