The authentication of food products is critically important in a global economy in public-health and economic terms The specific aims of this study were to evaluate the application of full spectrum and NIR spectroscopy and to evaluate the adoption of PLS and LS-SVM models to accomplish a rapid and non invasive quantification of the two common adulterants flour and mungbean powder in Spirulina powder The results showed that using all treatment sets only the LS-SVM models were adequate in predicting either adulterant under both full spectra and NIR spectra The use of NIR spectra would allow detection of adulterants even when masked by food dyes Design value analysis indicated that the benefits per unit cost of applying the NIR spectra to quantify adulterants in Spirulina powder significantly exceeded that of using full spectra and that the value of employing the LS SVM models under NIR spectra exceeded that of using the PLS models (C) 2010 Elsevier Ltd All rights reserved