This work investigates the application of the Solution-Enhanced Dispersion by supercritical fluids technique for the precipitation of beta-carotene. The effect of pressure (8.0-12.0 MPa), temperature (293-313 K) anti-solvent flow rate (20-40 mL/min), solution flow rate (1-4 mL/min) and concentration of beta-carotene in the dichloromethane solution (4 and 8 mg/mL) on the precipitation yield, particle morphology and particle size and size distribution was examined. Precipitated powders presented mean particle size varying from 3.2 mu m to 96.8 mu m with morphology of beta-carotene microparticles changing from plate-like to leaf-like particles. The statistical analysis of the experimental results revealed that pressure, organic solution concentration and CO2 flow rate had a significant effect on particle size. The precipitation yield was observed to be within the range of 71-94% and was statistically influenced by system temperature and pressure, and anti-solvent flow rate. (C) 2009 Elsevier Ltd. All rights reserved.